9+ tet-plko-puro vector

99100 based on 29 PubMed citations. We found 97 results for.

Construction Of A Single Lentiviral Vector Containing Tetracycline Inducible Alb Upa For Transduction Of Upa Expression In Murine Hepatocytes Plos One

Vector Type Resistance Marker Bacterial Resistance Source Sequence Available.

. Our primary modification was to shrink the stuffer. Digital collection of empty plasmid backbones from publications and commercially available sources. Addgene inc tet plko puro vector Tet Plko Puro Vector supplied by Addgene inc used in various techniques.

ZERO BIAS - scores. 99100 based on 16 PubMed citations. Addgene inc plko tet puro vector Plko Tet Puro Vector supplied by Addgene inc used in various techniques.

Ef Integrative Genomics Minggu 23. Vector Type Resistance Marker Bacterial Resistance Source Sequence Available. Gain unparalleled visibility of your plasmids DNA and protein.

ZERO BIAS - scores. The shRNAs used for PARG and HPF1 knockdown were cloned into the lentiviral vector Tet-pLKO-puro Addgene. The Tet-pLKO-Puro vector is a potentially powerful tool but the process of designing and cloning shRNAs into the vector is not without challenge.

In an effort to improve. Ligate 50 ng of digested pLKO1 TRC-cloning vector with 200 ng of annealed oligos from the previous steps. Transform 1-2 μL of ligation mix into.

Incubate at 16oC over night. Sign Up for Our Newsletter. SnapGene Viewer is free software that allows molecular biologists to create browse and share richly annotated sequence files.

In this study a single-vector lentiviral Tet-inducible shRNA system pLKO-Tet-On was generated to allow for the rapid generation of multiple stable cell lines with regulatable. 9 tet-plko-puro vector. First we modified the Tet-pLKO-Puro vector to make it easy EZ for molecular cloning EZ-Tet-pLKO-Puro.

Also Lipo2000 method has been done on multiple occasions with GFP plasmid as a control. So I am rather surprised that the packaging method is not working for Tet-pLKO-Puro.

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